TABLE 4.
Mass spectrometry data, obtained from analysis by tandem quadrupole time-of-flight MS, for identified protein spots from 2-D gel electrophoresis
| Spot no.a | Protein ID | Ion scoreb | MS/MS peptide data |
|---|---|---|---|
| 5 | GroEL2 | 39 | QIAFNSGLEPGVVAEK |
| 78 | DETTIVEGAGDTDAIAGR | ||
| 27 | SALQNAASIAGLFLRREAVVADKPEK | ||
| 32 | VIGAGKPLLIIAEDVEGEALSTLVVNK | ||
| 7 | PE_PGRS6a | 14 | AGAAGG |
| 2 | AGGNGG | ||
| 11 | GVGGVGG | ||
| 9 | DSGGDGG | ||
| 17 | LFGNGG | ||
| 13 | DSGGDGG | ||
| 9 | LFGNGG | ||
| 20 | GAGGFGQ | ||
| 12 | GAGGFGQ | ||
| 12 | GAGGFGQ | ||
| 12 | GAGGFGQ | ||
| 20 | GAGGFGQ | ||
| 14 | AQQYQ | ||
| 8 | mdh | 96 | VAVTGAAGQIGYSLLFR |
| 11 | Rv1265 | 53 | QVLDTLVDAGVAR |
| 27 | LPGLESPEEESAAR |
a
Numbers correspond to protein spots from shaking low-oxygen 2-D gel profiles (Fig 2).
b
Extensive homology indicated by ion score: GroEL2 > 26; PE_PGRS6a (total score) > 48; mdh > 25; Rv1265 > 43.