TABLE 5.
Quantitation of cAMP-induced proteins from BCG cultures grown shaking in 1.3% O2, 5% CO2
| Spot no.a | Rv ID | Protein ID | Protein function/category | Fold induction
|
||
|---|---|---|---|---|---|---|
| Protein | BCG mRNAb | Mtb mRNAc | ||||
| 1 | PE_PGRS | PE/PE_PGRS superfamily | 7.9 ± 1.3 | |||
| 2 | 3.1 ± 0.3 | |||||
| 3 | 4.3d | |||||
| 4 | 5.5d | |||||
| 5 | Rv0440 | GroEL2 | Heat shock protein/chaperone | 10.7 ± 0.7 | 2.3 | 3.2 |
| 6 | Rv2971 | Rv2971 | 2,5 Diketo-d-gluconic acid reductase | 3.4 ± 0.1 | 4.4 | 2.6 |
| 7 | Rv0532 | PE_PGRS6a | PE/PE_PGRS superfamily | 24.4 ± 2.6 | 3.0 | 2.0 |
| 8 | Rv1220 | Mdh | MDH | 6.1 ± 0.2 | 5.7 | 3.6 |
| Rv0440 | GroEL2 | Heat shock protein/chaperone | 6.1 ± 0.2 | 2.3 | 3.2 | |
| 9 | 3.0 ± 0.4 | |||||
| 10 | 4.4 ± 1.3 | |||||
| 11 | Rv1265 | Rv1265 | Unknown function/upregulated in macrophages | 8.4 ± 0.7 | 8.3 | 3.8 |
| 12 | 5.8d | |||||
a
Numbers correspond to protein spots from shaking low-oxygen 2-D gel profiles (Fig 2).
b
Fold levels determined by semiquantitative RT-PCR on BCG RNA.
c
Fold levels determined by semiquantitative RT-PCR on Mtb H37Rv RNA.
d
Only one round of quantitation was done for these spots, due to localized streaking on 2-D gel profiles.