TABLE 1.
Strains used
| Identifying genotype of strain | Characteristics | Reference and/or source |
|---|---|---|
| BS40 (dnaE+) | metB metD Δ(proAB lac) rpsL (Smr) | 31 |
| dnaE74 (BS40dnaE74) | metB metD Δ(proAB lac)dnaE74 rpsL (Smr) | 30 |
| Δthy | λ−thyA748::Tn10 rph-1 deo77 | E. coli Genetic Stock Center (K. B. Low) via H. Engelberg-Kulka |
| BS40Δthy | metB metD Δ(proAB lac) rpsL (Smr) thyA748::Tn1 | This paper; from Δthy by P1 transduction |
| dnaE74 cydA85 Tetr | metB Δ(proAB lac) rpsL (Smr) dnaE74 cydA85 nadA57::Tn10 | 29 |
| dnaE+cydA85 Tetr | metB metD Δ(proAB lac) rpsL (Smr) cydA85 nadA57::Tn10 | 29 |
| cydA85 Tets | metB metD Δ(proAB lac) rpsL (Smr) cydA85 | This paper; by selection (19) |
| Δthy cydA85 mutant | thyA748::Tn10 metB metD Δ(proAB lac) rpsL (Smr) cydA85 | This paper; from Δthy by transduction |
| dnaE74/pBR322 | metB Δ(proAB lac) dnaE74 (Ts) rpsL/pBR322 | By CaCl2 transformation (29) |
| dnaE74/pTK1 | metB Δ(proAB lac) dnaE74 (Ts) rpsL/pTK-1 | By CaCl2 transformation with plasmid from R. Gennis (29) |
| dnaE74/pcyO | metB Δ(proAB lac) dnaE74(Ts) rpsL/pcyO | This paper; by CaCl2 transformation with plasmid from R. Gennis |