TABLE 2.
Protective effect of the cydA85 mutation in minimal and LB mediuma
| Mutation | Survival (N/N0) after 6 h at 37°C in
|
|
|---|---|---|
| Minimal medium | LB medium | |
| Δthy | 0.023 | 0.00049 |
| Δthy cydA85 mutant | 0.00083 | 3.6 |
a
Strain Δ(thy cydA85) was grown for 23 h at 27°C in 10 ml of minimal medium. The cells were harvested, washed, and resuspended in 2 ml of PBS. Ten microliters of suspension was added to 10 ml of the medium indicated and was incubated with shaking. Cultures were diluted and plated for viability count on LB plates incubated at 27°C.