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. 2023 Dec 7;14:8088. doi: 10.1038/s41467-023-43975-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a RT–qPCR analysis of MPL1 mRNA expression levels in different tissues of WT (cv. CDC Frontiers) plants. b RT–qPCR analysis of MPL1 mRNA expression levels at different leaf developmental stages. M ~ P3: SAM with P1-P3 leaf primordia; P4-P8: leaf primordia at indicated developmental stages. c RT–qPCR analysis of MPL1 mRNA expression levels in different portions of the P4–P8 leaf primordia. The different leaf portions are illustrated to the right with the dotted line showing the site of dissection. PP, Proximal Portion; DP, Distal Portion. Cyan asterisk indicates the LL primordia. Data shows mean ± SD of 4 biological replicates in (a–c). The value above columns in (a) represents the p-value estimated using the two-sided unpaired Student’s t test. Different letters above bars in (b and c) indicate significant differences using the two-sided unpaired Student’s t test (p < 0.05). RNA in situ hybridization of MPL1. Shown are single longitudinal (d, e) and two serial transverse (f) sections of the SAM with several leaf primordia, and single (g–k) and serial (l, m) longitudinal sections of the leaf primordia at the indicated developmental stages. Similar results were obtained from three independent experiments. St, Stipule; LL: Lateral leaflet; TL: Terminal leaflet. Scale bars, 50 μm. n Diagrams of compound leaf primordia at successive stages of ontogeny and the spatio-temporal expression pattern of MPL1 (purple). Source data for Fig. 4a–c are provided as a Source Data file.