Skip to main content
. 2023 Dec 7;14:8088. doi: 10.1038/s41467-023-43975-9

Fig. 5. Expression patterns of KNOXI genes and CaLFY in the vegetative shoot meristem of chickpea.

Fig. 5

RNA in situ hybridization with CaSTMa (a, b), CaBP1(c, d) or CaLFY (e, f) specific probes on longitudinal (a, c, e) and transverse (b, d, f) sections of 4-week-old WT vegetative-shoots. Similar results were obtained from three independent experiments. Scale bars, 50 μm. g RT–qPCR analysis of CaLFY mRNA expression levels at different leaf developmental stages. Data shows mean ± SD of 4 biological replicates. Different letters above bars indicate significant differences using the two-sided unpaired Student’s t test (p < 0.05). h RT–qPCR analysis of CaLFY expression in different portions of the P5 leaf primordia of WT. PP, Proximal Portion; DP, Distal Portion. Data shows mean ± SD of 4 biological replicates. The value above column represents the p-value estimated by the two-sided unpaired Student’s t test. i A schematic illustration of CaSTMa and CaBP1 expression pattern in the shoot apex. j A schematic illustration of CaLFY expression pattern in the shoot apex. Source data for Fig. 5g, h are provided as a Source Data file.