Table 1.
Characterization of non-modified aSyn antibodies using recombinant synuclein proteins and peptides.
| Antibody | Epitope | Species reactivity | bSyn detection | gSyn detection | Other amyloidogenic protein reactivity | Neighboring PTM sensitivity | Conformational selectivity | |
|---|---|---|---|---|---|---|---|---|
| WB | DB | |||||||
| LASH-EGT403 | 1–5 | hu, mus | + | + | – | – | m/o/f | m/o/f |
| 5B10-A12 | 1–10 | hu, mus | – | – | – |
 M5 |
m/o/f | m/o/f |
| LASH-EGTNter | 1–20 | hu, mus | + | – | – | – | m/o/f | m/o/f |
| LASH-BL 34–45 | 34–45 | hu, mus | + | – | – |
 Y39 |
m/f | m/o/f |
| LASH-BL 80–96 | 80–96 | hu, mus | – | – | – | – | m/o/f | m/o/f |
| BD SYN-1 | 91–99 | hu, mus | – | – | – | – | m/o/f | m/o/f |
| BL 4B12 | 103–108 | hu | – | – | – | – | m/o/f | m/o/f |
| 2F10-E12 | 110–115 | hu, mus | – | – | – | – | m/o/f | m/o/f |
| AB LB509 | 115–122 | hu | – | – | – |
 P120-Y125 |
m/o/f | m/o/f |
| 7H10-E12 | 115–125 | hu | – | – | – | pY125 | m/o/f | m/o/f |
| LASH-BL 117–122 | 117–122 | hu | – | – | – |
 P120-Y125 |
m/o/f | m/o/f |
| LASH-BL A15127A | 120–122 | hu | – | – | – | – | m/o/f | m/o/f |
| 4E9-G10 | 120–125 | hu | – | – | – | pY125 | m/o/f | m/o/f |
| 1F10-B12 | 121–125 | hu | – | – | – | – | m/o/f | m/o/f |
| 4E9-C12 | 121–132 | hu | – | – | – | pY125 | m/o/f | m/o/f |
| 2C4-B12 | 123–125 | hu | – | – | – | pY125 | m/o/f | m/o/f |
| 6B2-D12 | 126–132 | hu, mus | – | – | +/TDP-43 | pS129 | m/o/f | m/o/f |
| AB 134–138 | 134–138 | hu, mus | + | – | – |
 D135 |
m/o/f | m/o/f |
= truncation, aSyn alpha-synuclein, bSyn beta-synuclein, DB dot blot, f fibril, gSyn gamma-synuclein, hu human, m monomer, mus mouse, o oligomer, PTM post-translational modification, TDP-43 transactive response DNA-binding protein 43 kDa, WB Western blot.