Table 4.
A summary of the mouse aSyn-reactive antibody detection patterns of exogenous PFFs in aSyn KO neurons, newly formed aggregates in PFF-seeded WT neurons and mouse brain tissues.
| Antibody | Epitope | aSyn KO neurons | PFF seeding model: WT primary neurons | PFF seeding model: WT mouse brain tissue | |||
|---|---|---|---|---|---|---|---|
| PFF detection - ICC | PFF detection - WB | Positivity | aSyn pS129* overlap | Positivity | aSyn pS129* overlap | ||
| LASH-EGT403 | 1–5 | + | + | + | – | – | – |
| 5B10-A12 | 1–10 | + | + | + | + | + | + |
| LASH-EGTNter | 1–20 | + | + | + | + | + | + |
| LASH-BL 34–45 | 34–45 | + | + | + | + | + | – |
| LASH-BL 80–96 | 80–96 | + | + | + | – | + | – |
| BD SYN-1 | 91–99 | + | + | + | – | + | – |
| 2F10-E12 | 110–115 | + | + | + | – | + | + |
| 6B2-D12 | 126–132 | + | – | + | – | + | – |
| AB 134–138 | 134–138 | + | + | + | + | + | + |
| LASH-EGT pY39 | pY39 | – | – | + | – | + | – |
| AB EP1536Y | pS129 | – | – | + | + | + | + |
| LASH-EGT pS129 | pS129 | – | – | + | + | + | + |
| AB pY133 | pY133 | – | – | + | – | – | – |
| AB pY136 | pY136 | – | – | + | – | – | – |
| LASH-EGT nY39 | nY39 | – | – | – | – | – | – |
| 5E1-G8 | nY39 |
+ (non-specific) |
– |
+ (non-specific) |
– | – | – |
| 5E1-C10 | nY39 |
+ (non-specific) |
– |
+ (non-specific) |
– | – | – |
aSyn alpha-synuclein, ICC immunocytochemistry, KO knockout, PFF pre-formed fibril, WB Western blot, WT wild-type.
*aSyn pS129 revealed by BL 81A mouse monoclonal and AB MJF-R13 rabbit monoclonal antibodies.