FIG. 1.

TLC analysis of the lipid intermediates from E. coli and M. smegmatis. (A) Radiolabeled, intact lipid I from E. coli (lane 1) and M. smegmatis (lane 2) was loaded onto a Kieselgel 60 F₂₅₄ TLC plate, which was developed in CHCl₃-CH₃OH-H₂O-NH₄OH-CH₃COONH₄ (5.6:4.2:0.68:0.27:0.27) and subjected to autoradiography. (B) Radiolabeled muropeptides obtained from E. coli lipid I (lane 1) or M. smegmatis lipid I (lane 2) were loaded onto a Kieselgel 60 F₂₅₄ TLC plate, which was developed in isobutyric acid-1 M NH₄OH (5:3) and subjected to autoradiography.