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TLC analysis of the lipid moiety of lipid II synthesized by the membrane-cell wall fraction from M. smegmatis. Autoradiogram of the radiolabeled products obtained by acid hydrolysis of lipid II from M. smegmatis (A) and generated by acid hydrolysis of decaprenyl phosphate (B). The arrows indicate the migration positions of authentic heptaprenol (C35) and decaprenol (C50). Samples were applied to a C18 silica gel reverse-phase TLC plate and developed with CH3COCH3-CH3OH (9:1).
