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. 2023 Nov 1;129(12):2014–2024. doi: 10.1038/s41416-023-02476-8

Fig. 3. Viability of NSCLC cells post-transfection and IR exposure.

Fig. 3

a Immunoblot showing COMMD4 levels in NSCLC and control HBEC3-KT cells transfected with control siRNA or COMMD4 siRNA #2 and FLAG-H2BWT or FLAG-H2BMUT1. β-actin shows the loading and FLAG shows expression of the H2B constructs. b–e Clonogenic cell viability assays in H460, H1299 and CRL5889 NSCLC cells and HBEC3-KT cells transfected with control or COMMD4 siRNA #2 and FLAG-H2BWT or FLAG-H2BMUT1 or mock-transfected and treated with varying doses of irradiation (IR). Asterix (*) denotes P < 0.05, n.s; not significant. Error bars represent mean ± SD from three independent experiments.