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. 2023 Nov 1;129(12):2014–2024. doi: 10.1038/s41416-023-02476-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Immunofluorescence staining of H1299 NSCLC cells transfected with control or COMMD4 siRNA #2 and FLAG-H2BWT or FLAG-H2BMUT1 is shown. 48–72 h post-siRNA transfection and 24 h post-FLAG H2B transfection, cells were treated with 6 Gy IR and the recovery time points (h) post-IR treatment is shown. b, c Plot of γH2AX and MDC1 foci numbers for (a). d Immunofluorescence staining of HBEC3-KT control cells as in (a). e, f Plot of γH2AX and MDC1 foci numbers for (d). n.s; not significant, *; P < 0.05 and **; P < 0.005. Error bars represent mean ± SD from three independent experiments where 50 cells were quantified per condition. γH2AX (green), MDC1 (red) and DAPI shows the nucleus. Scale bar denotes 5 μm.