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Effect of Tris-HCl addition on surfactin production during B. subtilis growth. A TLC analysis showing the amounts of surfactin initially in the medium (lane 1), in the negative control at 3 h after addition of water (lane 2), and at 3 h after addition of Tris-HCl (lane 3) is presented. Lane 4 contains a surfactin standard. Sample volumes analyzed were specific to the total amount of cell protein in each sample.
