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. 2005 Apr 1;115(4):900–909. doi: 10.1172/JCI200523675

Figure 6.

Figure 6

Developmental analysis of Dsh/+ limbs. Expression of marker genes relevant for digit development was performed using whole-mount (A and B) and section (C) in situ hybridization. (A) Expression of Shh in the posterior limb region and downregulation at E12.5 showing no difference between WT and Dsh/+. At E12.5 (B), we find normal patterns of expression for all genes tested. At E13.5 and E14.5 (C), the majority of genes show an abnormal pattern. Note misexpression of Hh receptor patched (ptc) and downstream effectors Gli1–Gli3 (arrows). Gdf5 first shows lack of expression in the developing joints (E13.5) and is then (E14.5) expressed in a more longitudinal fashion in the area of the future metacarpophalangeal joints (arrow). Col2a1 (Collagen type II a1; Col2) is expressed throughout the phalangeal anlagen but is less pronounced in the distal regions of Dsh/+ digits. Lack of Col10a1 (Collagen type X a1; Col10) expression in digit anlagen demonstrates delay in chondrocyte differentiation in Dsh/+. Magnification, ×50 (A–C).