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Monocytes and neutrophils represent the major non-DC in the non-adherent fraction of day 8 GM-CSF cultures. (A–D) D8 GM-CSF BM cultures were stained for the indicated markers. Pre-gating was performed like in Fig. 10A. Cell types are indicated in red. Percentages within quadrants are shown in black or are in magenta when calculated for the whole culture cellularity. (E) CD117 expression is detectable on MHC IIneg progenitor cells but also on mature MHC IIhigh BM-MoDC.
