Figure 17.

Maturation status of differentiated iCD103-DC.

(A) iCD103-DC were harvested on d16 and stimulated in a 96-well F-bottom cell culture plate overnight in the presence or absence of CpG-1826 ODN (1μM). Representative flow cytometry dot plots showing the frequencies of MHC-II⁺CD86⁺ cells among live CD11c⁺B220⁻CD103⁺Clec9a⁺ cells. (B). The expression of CD40, CD80, CD86, and MHC-II were analyzed by flow cytometry among live CD11c⁺B220⁻CD103⁺Clec9a⁺ cells in the presence (solid red line) or absence (solid black line) of overnight CpG-1826 ODN stimulation. In this example, 2 different staining panels were used.