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HoxB8 MPP differentiate into cDC1, cDC2, and pDC.
HoxB8 MPP were differentiated into DC with Flt3L for 5 and 9 days (panels a and B, respectively) and analyzed by flow cytometry. cDC1: CD11c+ CD11blow/− XCR1+; cDC2: CD11c+ CD11b+ XCR1− and MHCIIlow/− B220+ pDC. Representative flow cytometry analysis is shown. MHCIIhigh B220− CD11c+ cDC and MHCIIlow/− B220+ pDC were separated and MHCIIhigh CD11c+ cDC were further divided into CD11blow/− XCR1+ cDC1 and CD11b+ XCR1− cDC2.
