Table 1.
Reagents, antibodies, chemicals, and solutions
| Reagent | Manufacturer | Ordering Number |
|---|---|---|
| Antibodies | ||
| B220 APC-Cy7 (RA3-6B2) | Biolegend | 103223 |
| CD11b BV510 (M1/70) | Biolegend | 101245 |
| CD11c PE-Cy7 (N418) | Biolegend | 117317 |
| CD115 APC (AFS98) | eBioscience | 17-1152-80 |
| CD117 (c-Kit) PE-Cy7 (ACK2) | eBioscience | 25-1172-82 |
| CD135 (Flt3) PerCP-Cy5.5 (A2F10) | eBioscience | 46-1351-82 |
| Gr1 BV421 (RB6-8C5) | Biolegend | 108433 |
| Gr1 PerCP-Cy5.5 (RB6-8C5) | eBioscience | 45-5931-80 |
| MHCII PerCP-Cy5.5 (M5/114.15.2) | Biolegend | 107625 |
| XCR1 BV421 (ZET) | Biolegend | 148216 |
| Chemicals & Solutions | ||
| β-estradiol (E2) | Sigma | E2758 |
| β-mercaptoethanol (β-Me) | Gibco | 31350010 |
| Calcium chloride (CaCl2) | Merck | 1.02382.0500 |
| Chondroitin sulfate sodium salt from shark cartilage (CSC) | Sigma | C4384 |
| Dimethysulfoxide (DMSO) | Sigma | D8418 |
| DMEM | Gibco | 41965039 |
| EDTA | Gibco | 15575-038 |
| Fetal bovine serum (FBS) | PAA | A01125-499 |
| Fetal bovine serum (FBS) | Gibco | 10270106 |
| Recombinant human Flt3-Ligand (Flt3L) | Peprotech | 300-19 |
| Recombinant murine SCF | Peprotech | 250-03 |
| Human IGF-1 long range | Sigma | 85580C |
| Recombinant mouse IL-6/IL-6R alpha protein chimera | R&D Systems | 9038-SR |
| HEPES | Sigma | H4034 |
| L-glutamine | Gibco | 25030081 |
| Pancoll human, density 1.077g/ml (Ficoll) | PAN-Biotech | P04-601000 |
| Penicillin/streptomycin | Gibco | 15140122 |
| Phosphate buffered saline (PBS) | Gibco | 10010023 |
| Polybrene (PB, Hexadimethrine bromide) | Sigma | H9268 |
| RPMI 1640 | Gibco | 31870025 |
| Sodium chloride (NaCl) | Merck | 7647-15-5 |
| Sodium phosphate dibasic (Na2HPO4) | Merck | 7558-79-4 |