Table 11.
Fluorochrome-conjugated antibodies used for phenotypic identification of differentiated DC subsets
| Antibody | |||
|---|---|---|---|
| Fluorochrome | Antigen | Clone | Manufacturer |
| FITC | CD3 | SK7 (Leu-4) | BD Biosciences |
| CD16 | 3G8 | BD Biosciences | |
| CD19 | 4G7 | BD Biosciences | |
| CD20 | L27 | BD Biosciences | |
| CD34 | 8G12 | BD Biosciences | |
| CD56 | NCAM16.2 | BD Biosciences | |
| APC | CD303/CD304 | 201A/12C2 | BioLegend |
| APC-Cy7 | CD45 | 2D1 | BD Biosciences |
| AF700 | CD11c | B-ly6 | BD Biosciences |
| BV421 | CD123 | 6H6 | BioLegend |
| BV510 | CD141 | 1A4 | BioLegend |
| BV650 | CD14 | M5E2 | BioLegend |
| BV711 | CD163 | GHI/61 | BioLegend |
| BV785 | HLA-DR | L243 | BioLegend |
| PE | CLEC9A | 8F9 | BioLegend |
| PE-CF594 | CD2 | TS1/8 | BioLegend |
| PE-Cy7 | CD1c | L161 | BioLegend |
| BUV395 | CD15 | HI98 | BD Biosciences |
| DAPI | live/dead | Sigma | |