Table 6.
iCD103-DC generation
| Reagent or resource | Company | Catalog |
|---|---|---|
| RPMI 1640 | Thermo Fisher Scientific | 61870 |
| Fetal calf serum (FCS) heat inactivated | Biochrom | S0115 |
| 2-Mercaptoethanol | Gibco | 31350 |
| Penicillin/streptomycin | Biochrom/Merck | A2213 |
| DPBS | Sigma | D8537 |
| Murine GM-CSF culture supernatant | Made in-house | N/A |
| Murine FLT3L-flag culture supernatant | Made in-house | N/A |
| Mouse GM-CSF duoset ELISA | R&D | DY415 |
| Mouse FLT3L duoset ELISA | R&D | DY427 |
| Petri dishes 10 cm, sterile | Sarstedt | 821473001 |
| 50 ml tubes | Sarstedt | 62547254 |
| Cluster tubes (FACS) polypropylene 1.2 ml | Corning | 4401 |
| 96-well cell culture plate, PS, F-bottom | Greiner Bio-one | 655180 |
| ODN 1826-TLR9 ligand (Class B CpG) | Invivogen | Tlrl-1826-5 |
| Trypan Blue stain | Thermo Fisher Scientific | 15250061 |