FIG. 5.

Outer membrane profiles of bacterial strains which carry the iroN gene (lanes 3, 5, and 6) or in which iroN is lacking (lanes 7 and 8) or inactivated (lane 2). The position of IroN is indicated by an arrow. Positions and sizes of bands from standard proteins are indicated (lanes 1 and 4). (A) SDS-PAGE of outer membrane preparations of S. enterica serotype Typhimurium AJB64 (lane 2) and IR715 (lane 3) grown under iron limitation. (B) Outer membrane profiles of E. coli H5058 (lanes 7 and 8) and H5058(pTY908) (lanes 5 and 6) grown in LB supplemented with 0.2 mM 2,2′-dipyridyl (lanes 6 and 8) or 0.04 mM FeSO₄ (lanes 5 and 7).