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. Author manuscript; available in PMC: 2023 Dec 8.
Published in final edited form as: Eur J Neurosci. 2016 Feb 5;43(5):653–661. doi: 10.1111/ejn.13163

Figure 2.

Figure 2

Distribution of CTR, RAMP1 and RAMP3 mRNAs in single cell of amylin-treated rats. (a) Nissl staining of rat AP at 20x. Representative image showing the LCM laser (blue cross) in the act of collecting a single neuron (red circle with number 1). The green circle represents the ultraviolet laser cutting spot. (b) In randomly picked individual neurons (n=8–10 cells per rat) from the AP of amylin-treated (20 μg/kg) male Wistar rats (n=4), 62% of the randomly collected single cells are CTRa−; most of the CTRa− cells are also RAMP1− and RAMP3− (53%); 6% of all cells are CTRa−, RAMP1+ and RAMP3− and 3% of all cells are CTRa−, RAMP1− and RAMP3+. The remaining 38% of individual neurons are CTRa+, and the 25% of them co-express CTRa and both RAMP1 and RAMP3. The remaining cells co-expressed CTRa and only RAMP1 (3%) or CTRa and only RAMP3 (10%). RAMP1 and RAMP3 transcripts are preferentially expressed in CTRa positive vs CTRa negative neurons; Chi-square test, *** P < 0.001.