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. Author manuscript; available in PMC: 2023 Dec 8.
Published in final edited form as: Eur J Neurosci. 2016 Feb 5;43(5):653–661. doi: 10.1111/ejn.13163

Figure 6.

Figure 6

Quantitative analysis of LepRb mRNA levels in area postrema single neurons. Single cells (n=4 rat per group; 9–10 cells per rat) that were positive for all components of the functional AMY (AMY+) were analysed for the presence or absence of LepRb mRNA. (a) In vehicle-treated rats, 52% of the single-cells co-expressed AMY and LepR mRNAs. (b) In amylin-treated rats, 30% of the Fos positive single-cells that were AMY+ also co-expressed LepRb mRNA. (c) Quantitative analysis of LepRb mRNA levels; GAPDH was used as housekeeping gene and mRNA expression is shown as fold change. LepRb transcript levels were significantly up-regulated in AMY+ cells from amylin-treated compared to vehicle treated rats (*P < 0.05). Data shown as mean±SEM.