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. 2023 Nov 24;14:1301154. doi: 10.3389/fphar.2023.1301154

TABLE 5.

The antimicrobial effects of Anchusa strigosa.

Extract Dose Experimental model Organisms Main results References
Antibacterial
Essential oil and fixed oil of the flowers Dose range: 10–500 μg/mL Minimal active dose: - Essential oil:200 μg/mL - fixed oil: 500 μg/mL Method: Agar disk diffusion Bacillus subtilis - Essential oil was more active than fixed oil - Essential oil showed potent activity specially against Pseudomouos aeruginsa, Proteus sp. and Streptococcus faecalis - Fixed oil showed strong activity against P. aeruginosa, Klebsiella sp. and, Proteus sp. Al-Salihi F. et al. (2009)
Positive control: Enterobacter sp.
- Pencillin (10 units) Escherichia coli
- Ampicillin (10 mg) Klebsiella sp.
- Carbenicillin (100 mg) Proteus sp.
- Chloramphenicol (30 mg) Pseudomouos aeruginsa
- Nitrofurantion (300 mg) Staphylococcus aureus Staphyloccus epidermidis
- Nalidixic acid (30 mg) Streptococcus faecalis
- Cephalexin (30 mg) Streptococcus viridance
- Tetracycline (30 mg)
- Kanamycin (30 mg)
- Erythromycin (15 mg)
Total lipids of flowers using n-hexane Dose range: 0.01–10 mg/mL Minimal active dose: 0.5 mg/mL Method: Agar disk diffusion Bacillus subtilis - Strong antibacterial activity. - More effective against gram-positive bacteria. - Most susceptible Gram-positive bacteria was Streptococcus faecalis.- Most susceptible gram-negative bacteria was Pseudomonas aeruoginosa. Al-Salihi F. et al. (2007)
Positive control: Enterobacter sp.
- Pencillin (10 units) Escherichia coli
- Ampicillin (10 mg) Klebsiella sp.
- Carbenicillin (100 mg) Proteus sp.
- Chloramphenicol (30 mg) Pseudomonas aeruoginosa
- Nitrofurantion (300 mg) Staphylococcus aureus
- Nalidixic acid (30 mg) Staphylococcus epidermidis
- Cephalexin (30 mg) Streptococcus faecalis
- Tetracycline (30 mg) Streptococcus viridians
- Kanamycin (30 mg)
- Erythromycin (15 mg)
Ethanolic extract of aerial parts 10 mg/mL Method: Agar disk diffusion Escherichia coli - Strong antibacterial activity against Staphylococcus aureus Ali-Shtayeh et al. (1998)
Positive control: Klebsiella pneumonia
- Ampicillin Proteus vulgaris
- Penicillin-G Pseudomonas aeruginosa Staphylococcus aureus
- Gentamicin
Ethanolic extract of leaves and flowers 0.1 mg/mL Method: WST-1 assay Acinetobacter baumannii - Strong activity against drug-resistant bacteria Yarmolinsky et al. (2022)
Escherichia coli
Klebsiella pneumoniae
Salmonella enteritidis Serratia marcescens
Aqueous extract of aerial parts 10 mg/mL Method: Agar disk diffusion Escherichia coli - Good antibacterial activity against Proteus vulgaris Ali-Shtayeh et al. (1998)
Positive control: Klebsiella pneumonia
- Ampicillin Proteus vulgaris
- Penicillin-G Pseudomonas aeruginosa Staphylococcus aureus
- Gentamicin
Aqueous extract of stems, leaves, and roots Not specified Method: Agar disk diffusion Aeromonas hydrophila - Strong antibacterial effect against Photobacterium damselae Abutbul et al. (2005)
Photobacterium damselae
Streptococcus iniae
Vibrio alginolyticus
Antifungal
Ethanolic extract of aerial parts 10 mg/mL Method: Agar disk diffusion Candida albicans - Good antifungal activity Ali-Shtayeh et al. (1998)
Positive control:
- Nystatin
Aqueous extract of aerial parts 15 μg/mL Method: Agar dilution Microsporum canis - Good inhibitory effect against the three tested dermatophytes Ali-Shtayeh and Abu Ghdeib (1999)
Positive control: Trichophyton mentagrophytes
- Griseofulvin Trichophyton violaceum