Figure 1.

Intraplatelet calcium mobilization is responsible for the time-dependent incorporation of platelets in thrombi. (A) Representative images of the course of platelet calcium flux over time. Wild-type platelets were purified, labeled with Fura-2 AM, and reinjected into a recipient mouse through the jugular vein (1% of the total platelets were injected). Platelets are labeled in green, and the calcium flow inside the platelets is depicted in yellow. the width of the artery was typically 20 μm. (B) Quantitative analysis of the relationship between the calcium flux mobilization in platelets and their detachment from the thrombus (n = 18 events, 14 thrombi, 3 mice). (C) Quantitative analysis of the time for platelets to mobilize calcium in the thrombus (n = 22 events, 14 thrombi, 3 mice). (D) Graph represents the duration of the platelets inside the thrombus according to their calcium mobilization state (n = 17 events, 14 thrombi, 3 mice). Data are shown as medians, and statistics were performed using a one-way analysis of variance with Mann–Whitney U-test at 95% CI (E) Graph represents the correlation between the duration of platelets in the growing thrombus depending on the duration of the calcium mobilization (n = 17 events, 14 thrombi, 3 mice). ∗∗ P <.01.