TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Strains | ||
| BW545 | Δ(lacU)169 rpsL | Laboratory collection |
| VJS1779 | RK4353 moe-251::Tn10d(Tc) | V. Stewart |
| VJS1783 | RK4353 moe-255::Tn10d(Tc) | V. Stewart |
| C26 | gal-26 chlE5 (moeA5) supE42 | CGSC 4459 |
| JT1 | cysN-Km | CGSC 7057 |
| JM73 | cysJ90 | CGSC 5748 |
| SE1581 | BW545 moeA101 | This study |
| SE1588 | BW545 moeA103 | This study |
| SE1905 | SE1588 zbh::Tn10 | This study |
| SE1595 | BW545 modC118 | 25 |
| SE2147 | AH69 zbh::Tn10 | This study |
| AH1 | SE1588 cysN-Km | SE1588 × P1 (JT1) |
| AH8 | JM73 moeA103 zbh::Tn10 | JM73 × P1 (SE1905) |
| AH10 | JT1 moeA103 zbh::Tn10 | JT1 × P1 (SE1905) |
| AH30 | moeB101-Km | This study |
| AH69 | moeA113 ΔzbiK-Km | This study |
| AH131 | BW545 cysN-Km | BW545 × P1 (JT1) |
| AH134 | AH131 moeA113 | AH131 × P1 (SE2147) |
| Plasmids | ||
| pACYC184 | Tcr Cmr cloning vector | 6 |
| pBR322 | Amr Tcr cloning vector | 4 |
| pMAK705 | rep(Ts) Cmr | 11 |
| pUC19 | AmrlacZ′ cloning vector | 35 |
| pUC4K | Kmr Amr | Pharmacia |
| pFGH1 | pACYC184 based, moeA+B+ | Laboratory collection |
| pAH1 | pUC19 based, moeA+B+ | This study |
| pAH2 | pUC19 based, moeA+B+ | This study |
| pAH3-1 | pUC19 based, (moeB-Km) Amr | This study |
| pAH6 | pUC19 based, ΔmoeA moeB+ | This study |
| pAH11 | pMAK705 based, (moeB-Km) rep(Ts) Cmr | This study |
| pAH20 | pUC19 based, moeA+ ΔmoeB | This study |
| pAH43 | pUC19 based, moeA113 moeB+ | This study |
| pAH44 | pAH43 zbiK-Km | This study |
| pAH55 | pMAK705 based, moeA113 moeB+ zbiK-Km | This study |