TABLE 3.
Strain | Relevant genotype | Enzyme activity (nmol min−1 mg of cell protein−1)
|
||||||
---|---|---|---|---|---|---|---|---|
FHL
|
Nitrate reductase
|
|||||||
No plasmid | pAH2 | pAH20 | pAH6 | No plasmid | pAH2 | pAH20 | ||
BW545 | Wild type | 230 | 220 | 220 | 170 | 650 | 730 | 620 |
SE1581 | <1 | 160 | 90 | NDb | 40 | 500 | 510 | |
SE1588 | <1 | 180 | 80 | <1 | 40 | 610 | 450 | |
AH30 | moeB101-Km | <1 | 150 | <1 | 135 | 40 | 600 | 40 |
Cultures were grown in LBG medium for FHL activity and LBGN medium for nitrate reductase activity.
ND, not determined.