TABLE 5.
Effect of sulfide on nitrate reductase activity produced by moeA mutantsa
Strain | Relevant genotype | Nitrate reductase activity (nmol min−1 mg of cell protein−1)
|
|||
---|---|---|---|---|---|
LBGN | LBGN + S2− | LSMN | LSMN + S2− | ||
BW545 | Wild type | 670 | 700 | 585 | 530 |
AH131 | cysN-Km | 630 | 725 | 570 | 625 |
AH69 | moeA113 | 40 | 380 | 320 | 300 |
AH1 | moeA103 cysN-Km | 55 | 400 | 65 | 360 |
AH134 | moeA113 cysN-Km | 50 | 280 | 40 | 275 |
Sodium molybdate concentrations were 1 mM in LBGN and 0.1 mM in LSMN. Sodium sulfide concentrations were 2 mM in LBGN and 0.2 mM in LSMN.