FIG. 4.
Temperature-dependent processing of the M. thermophila β prosubunit. Purified α7 rings and β prosubunits were combined in subunit equimolar amounts (15 μM, final concentration) and incubated for 60 min at 16°C (lane 1), 21°C (lane 2), 30°C (lane 3), 37°C (lane 4), 42°C (lane 5), 50°C (lane 6), 55°C (lane 7), 60°C (lane 8), or 70°C (lane 9). A sample of the protein mixture (1.1 μg) was analyzed by SDS-PAGE by using 12% polyacrylamide. Proteins were stained with Coomassie blue R-250. Molecular size standards are indicated on the right.