TABLE 4.
Protein degradation activity of the 20S proteasome from M. thermophila
Substrate | Sp acta |
---|---|
β-Casein | 31.0 |
Dephosphorylated β-casein | 31.0 |
Lysozyme | 52.0 |
Bovine serum albumin | 2.0 |
Phosphorylase b | 0.1 |
Expressed as nanomoles of leucine equivalents per hour per milligram of protein. The assay (60°C) contained 1.0 mg of substrate/ml and 0.067 mg of the 20S proteasome produced in E. coli per ml.