Table 4.

Phenotypic and genotypic characterization of mcr positive E. coli from broiler chicken and farm environment (n = 48)

Isolate ID	Source	ST	Resistant Antibiotics	Colistin MIC(µg/ml)	Phylogroup group	mcr and ESBL gene	Virulence gene
KBF1	Fecal	165	CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM	≥ 4	A	mcr-4, mcr-6, CTX	ast, iss,irp2
F6(2)	Fecal	605	CIP,AMP,CHL,TET,SXT,STR,NAL,GEN	≥ 4	A	mcr-1	ast,irp2
19D(2)	Evt swab	7506	CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM,GEN	≤ 2	B1	mcr-7	iss
BFd8H3	Food	1771	CIP,AMP,CHL,TET,SXT,STR,NAL,	≤ 2	A	mcr-8	ast,iss,irp2
ZC5B	cloacal	1771	CIP,AMP,CHL,TET,SXT,STR,NAL,	≤ 2	C	mcr-8	ast
B9H2	cloacal	155	CIP,AMP,CHL,TET,SXT,STR,	≤ 2	E	mcr-1	ast, iss,irp2,iucD
12B	cloacal	48	AMC,CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,	≤ 2	Clade I or II	mcr-1	ast, iss,irp2,iucD
3BA	cloacal	1703	CIP,AMP,CHL,TET,SXT,STR,NAL	≤ 2	A	mcr-1	ast,pap C
ZC4BN	cloacal	206	CIP,AMP,CHL,TET,STR,	≤ 2	C	mcr-8, SHV	ast
C39H4	cloacal	1771	CIP,AMP,CHL,TET,SXT,STR,NAL	≤ 2	A	mcr-8, CTX	ast,papC
YC1H2	cloacal	354	CIP,AMP,CHL,TET,SXT,STR,NAL,GEN	≥ 4	F	mcr-6	ast
Dr7H2	Water	1771	CIP,AMP,CHL,TET,SXT,STR,NAL,	≥ 4	A	mcr-8	irp2
E15(2)	Evt swab	206	CAZ,AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM, GEN	≥ 4	A	mcr-1	ast,tsh,iss
KB4A	cloacal	162	CIP,AMP,CHL,TET,SXT,STR,NAL,GEN	≤ 2	B1	mcr-9, CTX	ast,iss, iuc D
BFd5H4	Food	159	CIP,AMP,CHL,TET,SXT,STR,NAL,CXM,	≥ 4	C	mcr-1,mcr-5	ast,iss, iuc D
B8H4	cloacal	11,630	AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM	≤ 2	B1	mcr-6	ast, iuc D
B7H4	cloacal	38	CIP,AMP,CHL,TET,SXT,STR,NAL,GEN	≥ 4	D	mcr-7, mcr-1	ast,iss,papC,iucD
E43A	Evt swab	398	CIP,AMP,CHL,TET,SXT,STR,NAL,	≥ 4	F	mcr-6, CTX	ast,iss
Drw3	Water	206	CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CRO,	≥ 4	A	mcr-1, mcr-8, SHV	ast
Drw7	Water	1285	CIP, AMP,CHL,TET,SXT,STR,NAL,CXM,GEN	≥ 4	B1	mcr-1, SHV	_
WF5	Swage	1140	CAZ,AMC,CIP,AMP,CHL,TET,SXT,STR,NAL,CXM	≥ 4	D	mcr-7	iss,irp2
L25B	Litter	106	CAZ,AMC,CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM	≥ 4	D	mcr-1, SHV,CTX	irp2
Food_G	Food	43	AMP,CHL,TET,SXT,STR,NAL	≥ 4	C	mcr-1	_
7a	cloacal	_	CIP,IPM,AMP,CHL,TET,SXT,STR,NAL	≥ 4	D	mcr-6	_
B2H1	cloacal	_	CIP,AMP,CHL,TET,SXT,STR,NAL,	≥ 4	B2	mcr-6	_
BFood7H1	Food		CIP,AMP,CHL,TET,SXT,STR,NAL,CXM	≤ 2	A	mcr-7	_
Drw1	Water	_	CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,	≥ 4	D	mcr-8	_
Drw4	Water	_	CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CRO,	≥ 4	B1	mcr-8	_
E1	Evt swab	_	CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM,GEN	≥ 4	A	mcr-1	_
E21	Evt swab		CIP,AMP,CHL,TET,SXT,STR,	≤ 2	Unknown	mcr-7
E22	Evt swab		CIP, AMP,CHL,TET,SXT,STR,NAL,	≤ 2	A	mcr-1	_
E28	Evt swab		CIP,AMP,CHL,TET,SXT,STR,NAL,	≤ 2	E	mcr-1
EVT11	Evt swab		AMC,CIP,AMP,CHL,TET,SXT,STR,NAL,	≤ 2	A	mcr-7
EVT16	Evt swab	_	CIP,AMP,CHL,TET,SXT,STR,NAL,GEN	≥ 4	A	mcr-1	_
EVT21	Evt swab	_	CIP,AMP,CHL,TET,SXT,STR,ATM,NAL,	≥ 4	A	mcr-1	_
F1	Fecal	_	CIP,AMP,CHL,TET,SXT,STR,NAL,	≥ 4	A	mcr-7	_
L16	Litter	_	CIP,AMP,CHL,TET,SXT,STR,NAL,	≥ 4	A	mcr-1	_
L17	Litter	_	CIP,AMP,CHL,TET,SXT,STR,ATM,NAL	≥ 4	C	mcr-8	_
YC23H2	cloacal		CIP,AMP,CHL,TET,SXT,STR,	≤ 2	A	mcr-1	ast
ZC13H1	cloacal		CIP, AMP,CHL,TET,SXT,STR,NAL,GEN	≤ 2	A	mcr-1
ZC22H1	cloacal	_	AMP, CHL,TET,STR	≥ 4	B1	mcr-1	_
EVT13	Evt swab		AMC, CIP,AMP,CHL,TET,SXT,STR,NAL,CXM	≤ 2	A	mcr-1
Fecal	Fecal		CIP, AMP,CHL,TET,SXT,STR,CTX,NAL,CRO,CXM	≤ 2	Clade I or II	mcr-1
FoodB	Food	_	CAZ, MEM,CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM	≥ 4	A	mcr-1	_
FoodD	Food		AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM,GEN	≤ 2	A	mcr-1
11a	cloacal		AMC, CIP,AMP,CHL,TET,SXT,STR,CTX,NAL,CXM	≤ 2	A	mcr-6
E2	Evt swab	_	CIP, AMP, CHL,TET,SXT,STR,NAL,GEN	≥ 4	A	mcr-8	_
12a	cloacal		CAZ,CIP,AMP,CHL,TET,SXT,STR,CTX,ATM,NAL,CRO,CXM,GEN	≤ 2	A	mcr-6

ATM Aztreonam, CTX Cefotaxime, AMC Amoxicillin-clavulanic acid, CAZ Ceftazidime, CRO Ceftriaxone, SXT Trimethoprim-sulfamethoxazole, CHL Chloramphenicol, TET Tetracycline, IMP Imipenem, MEM Meropenem, CIP Ciprofloxacin, AMP Ampicillin, STR Streptomycin, NAL Nalidixic acid, CXM Cefuroxime, GEN Gentamicin