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. 2023 Dec 8;24:284. doi: 10.1186/s13059-023-03122-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — PML nuclear bodies in H3.3 K27M vs canonical H3.1 K27M mutated patient-derived glioma cells. Immunofluorescence of PML (red) and H3K27M (green) in A DIPG XIII and BT245 H3.3 K27M mutated patient-derived glioma cells compared to isogenic H3.3 WT (K27M KO) controls. B H3.1 K27M mutated patient-derived glioma cells: #1 (SU_DIPG_36), #2 (ICR_B184_2D), #3 (SU_DIPG_33), and #4 (SU_DIPG_4). Scale bar 2 µM. C Quantitation of PML-NBs per cell. 50 nuclei (n = 50) were counted from three independent immunofluorescence experiments per cell line. Dots represent counts per cell (WT (K27MKO); H3.3 K27M in DIPG XIII and BT245 cells; #1; #2; #3; #4 H3.1 K27M lines). 25th, median, and 75th percentiles are shown. H3.1 K27M (combined) shows an aggregate of all four H3.1 K27M cell lines. P-values were calculated using two-tailed Student’s t test (*P < 0.0001)