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. 2023 Dec 8;24:284. doi: 10.1186/s13059-023-03122-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PML knockdown inhibits differentiation in H3.3 WT (K27M KO) glioma cells. A Immunofluorescence of K27M (green) and PML (red) in DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M pediatric glioma cells (clone #1) with and without doxycycline-induced PML knockdown. Scale bar 2 µM. B Immunofluorescence staining of GFAP glial marker (green) and β-tubulin (red) of DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M glioma cells (clone #1) with and without PML knockdown, over 7 and 14 days of culture in differentiation media. C Western blots of DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M glioma cells overexpressing myc/flag tagged PML (denoted + PML) using anti-myc and actin antibodies. D Immunofluorescence staining of GFAP glial marker (green) and β-tubulin (red) of DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M glioma cells with and without PML overexpression, over 7 and 14 days of culture in differentiation media