FIG. 2.

Elution profile of the secondary cell wall polymers from B. stearothermophilus ATCC 12980, PV72/p6, and PV72/p2 after application to RP-HPLC as described previously (6). Only the secondary cell wall polymers from B. stearothermophilus PV72/p6 and PV72/p2 eluted as a single peak, showing that this material was homogeneous. In contrast to B. stearothermophilus ATCC 12980, which was grown in batch culture, B. stearothermophilus PV72/p6 and PV72/p2 were grown in continuous culture at constant specific growth rate. The absorption at 220 nm is plotted versus the elution time in minutes.