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. 1998 Mar;180(6):1488–1495. doi: 10.1128/jb.180.6.1488-1495.1998

FIG. 7.

FIG. 7

Lanes a and b, SDS-PAGE patterns of proteolytic cleavage fragments of the S-layer proteins from B. stearothermophilus PV72/p6 and ATCC 12980, respectively. Proteolytic cleavage fragments used for N-terminal sequencing are indicated by arrowheads. Lanes c and d, immunoblots obtained by applying the anti-ATCC 12980 antiserum to the proteolytically degraded S-layer proteins from B. stearothermophilus PV72/p6 and ATCC 12980, respectively. Four cleavage fragments of the S-layer protein from B. stearothermophilus PV72/p6 gave a weakly positive reaction with the anti-ATCC 12980 antiserum on immunoblots (lane c).