TABLE 3.
Cleavage fragments of the S-layer protein SbsA from B. stearothermophilus PV72/p6 produced with endoproteinase Glu-C
| Fragment | Mr according to SDS-PAGE | N-terminal sequence | Position of first amino acid on the mature SbsA | Mr of missing N/C-terminal fragmenta |
|---|---|---|---|---|
| A | 90,000 | ATDVAT | N terminus | ------/40,000 |
| B | 80,000 | ATDVAT | N terminus | ------/50,000 |
| C | 66,000 | AALTPK | 228 | 24,000/40,000 |
| D | 60,000 | AALTPK | 228 | 24,000/46,000 |
a
Based on the apparent molecular weight of the SbsA of 130,000 determined by SDS-PAGE.