Table 1.
Commercialized sperm sexing options for various species and their accuracy.
| Technique | Technique principle | Target specie | Cellular type | Accuracy (%) |
|---|---|---|---|---|
| Flow cytometry | X-sperm has more DNA content than Y-sperm, allowing them to be sorted | Bovine | X-sperm | 75% and 85–95% |
| Y-sperm | 75% and 85–95% | |||
| Caprine | X-sperm | 93% | ||
| Ovine | >90% | |||
| Sexcel sexed genetics™ | Gender ablation | Bovine | X-sperm | 85% |
| WholeMom kit (Nuri Science Inc.) | An antibody promotes the agglutination of the Y-sperm, slowing them down | Bovine | X-sperm | 90% (heifers) |
| Equine | Not specified | |||
| Caprine | Not specified | |||
| Ovine | Not specified | |||
| Canine | Not specified | |||
| Porcine | 70% (sows) | |||
| WholeMan kit (Nuri Science Inc.) | An additive enhances the motility of Y-sperm over that of X-sperm | Bovine | Y-sperm | 90% |
| EMLAB Genetics’ kits | An additive enhances the fertility and motility of X-sperm while slowing down the Y-sperm | Bovine | X-sperm | 75–90% (fresh semen) 70–75% (post-thaw semen) |
| Equine | 65–85% (fresh and cooled semen) 70–75% (post-thaw semen) | |||
| Caprine | 75–90% (fresh semen) 80–85% (post-thaw semen) | |||
| Ovine | 75–90% (fresh or post-thaw semen) | |||
| Canine | 75–85% (fresh, cooled, or post-thaw semen) | |||
| Porcine | 80–90% (fresh or post-thaw semen) | An additive enhances the fertility and motility of Y-sperm while slowing down the X-sperm | ||
| Bovine | Y-sperm | 75–90% (fresh semen) 75% (post-thaw semen) | ||
| Equine | 65–85% (fresh, cooled, or post-thaw semen) | |||
| Caprine | 75% (post-thaw semen) | |||
| Ovine | 75% (post-thaw semen) | |||
| Canine | 75–80% (fresh, cooled, or post-thaw semen) | |||
| Porcine | 80–90% (fresh or post-thaw semen) |