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. 2023 Dec 5;11:e16566. doi: 10.7717/peerj.16566

Figure 3. miR-4270 directly targets the 3′UTR of DNMT3A.

Figure 3

(A) Binding sequences of miR-4270 and the 3′UTR of DNMT3A. (B) Relative luciferase activities of miR-4270 and DNMT3A were tested by luciferase reporter gene assay, t-test. (C) The RIP test was conducted to assess the relative enrichment level of miR-4270 and DNMT3A in HCC cells, t-test. (D) The mRNA levels of miR-4270, and (E) DNMT3A in miR-4270 mimic or miR-4270 inhibitor transfected HCC cells were assessed by RT-qPCR, t-test. (F) Western blot evaluation of DNMT3A levels in miR-4270 mimic or miR-4270 inhibitor transfected HCC cells, t-test. (G) Online analysis of DNMT3A levels in HCC tissues through the GEPIA website. (H) Online analysis of DNMT3A in different clinical stages of HCC patients through the GEPIA website. (I) Online analysis of disease-free survival curves of DNMT3A patients through the GEPIA website. **P < 0.01. N = 6.