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. 2023 Dec 5;11:e16566. doi: 10.7717/peerj.16566

Figure 5. HGFAC overexpression could antagonize the facilitative effect of miR-4270 inhibitor on the malignant behavior of HCC cells.

Figure 5

(A) Western blot assessment of HGFAC levels in HepG2 cells transfected with miR-4270 inhibitor or/and pcDNA-HGFAC, t-test. (B) Proliferation of miR-4270 inhibitor or/and pcDNA-HGFAC transfected HCC cells by CCK-8 assay, t-test and two-way ANOVA. (C) The invasion ability of miR-4270 inhibitor or/and pcDNA-HGFAC transfected HCC cells was assessed by transwell assay. Scale bar: 100 µm, t-test. (D) Annexin V-FITC/PE assay evaluation of the apoptotic ratio of HCC cells transfected with miR-4270 inhibitor or/and pcDNA-HGFAC, t-test. (E) Flow cytometry evaluation of the cycle distribution of miR-4270 inhibitor or/and pcDNA-HGFAC transfected HCC cells t-test. *P < 0.05, **P < 0.01. N = 6.