TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | (φ80dlacZΔM15) ΔlacU169 recA1 endA1 hisdR17 supE44 thi-1 gyrA96 relA1 | 13 |
| DH5αphe | DH5α phe::Tn10dCm | 7 |
| HB101 | lacY1 galK2 supE44 ara14 proA2 rpsL20 recA13 xyl-5 mtl-1 hisdS20 mcrB mrr | 8 |
| R. sphaeroides | ||
| 2.4.1 | Wild type | W. Sistrom |
| JZ1678 | ΔfnrL::ΩKmr | 30 |
| JZ1692 | ΔfnrL::ΩSpr/Str | 30 |
| JZ1844 | ppsR+ ppsR::ΩKmr ΔfnrL::ΩSpr/Str | This study |
| JZ1846 | ppsR::ΩKmr ΔfnrL::ΩSpr/Str | This study |
| JZ1847 | ppsR::ΩKmr ΔfnrL::ΩSpr/Str | This study |
| Plasmids | ||
| pCF200 | RSF1010 derivative carrying a puc::lacZ transcriptional fusion (Fig. 1) | 16 |
| pCF250 | RSF1010 derivative carrying a puc::lacZ transcriptional fusion (Fig. 1) | 16 |
| pRK415 | Mob+ Tcr IncP | 15 |
| pRK2013 | Kmr IncP1 ColE1 Mob | 8 |
| pLK-1 | RSF1010 derivative carrying a puc::aph transcriptional fusion | 6 |
| pUI1649 | pRK415 derivative, prrB+ | 6 |
| pUI1650 | pRK415 derivative, prrB78 | 6 |
| pUI1650* | pRK415 derivative, prrB78* | This study |
| pUI1970 | pRK415 derivative, fnrL+ | 32 |
| p714SmH::Kmr::mob | ppsR::ΩKmr | 12 |