Figure 2.

Needle-free injection system does not compromise virus infectivity in vitro. (A): Representative fluorescent images from Incucyte ZOOM^TM for MeV, HSV1, VACV, and VSV at 18 h post-infection from three different MOIs for each virus with respective quantifications below. Graphs depict the mean ± s.d. of four different images at 10X magnification taken every two hours for the total assay duration of 72 h. Data are representative of three independent experiments. (B): Representative immunofluorescent images of ReoV infectivity with 3 different titers (TCID₅₀). 48 h after infection with ReoV, HEK293T cells were fixed and labeled using immunofluorescence. (C): Representative Western blots of viral protein from HEK293T cells 12 h after infection with HSV1, VACV, VSV or ReoV.