TABLE 1.
Acinetobacter strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Acinetobacter strains | ||
| ADP1 | Wild type (strain BD413) | 32 |
| ADP6 | pcaG6 | 21 |
| ADP92 | pcaU1::ΩSmrSpcr | This study |
| ADP331 | ΔpcaU2, a 552-bp deletion between NcoI and MluI restriction sites in pcaUa | This study |
| ADP500 | ΔpcaB′DK′1 ΔcatD101::Kmr | 21 |
| ADP5126 | ΔpcaB′DK′1 ΔcatD101::KmrpcaP1 | 21 |
| ADP6126 | ΔcatD101::KmrpcaP1 | This study |
| Plasmids | ||
| pKOK6 | Contains promoterless lacZ-Kmr cassette for constructing transcriptional fusions | 35 |
| pHP45Ω | Contains ΩSmrSpcr cassette terminating transcription and translation | 51 |
| pRK415 | Tcrlacp/o | 33 |
| pUC18 | Aprlacp/o | 62 |
| pUC19 | Aprlacp/o | 62 |
| pWH660+ | Shuttle vector for transfer of genes between E. coli and Acinetobacter | 30 |
| pWH661+ | Xho site deleted from pWH660+ | This study |
| pZR1 | 11,062-bp EcoRI fragment containing pcaIJFBDKCHGquiBCX′ and inserted in pUC18 | 13 |
| pZR9 | 5,403-bp Sau3A fragment containing pcaU′-pcaIJFBD′ and inserted in pUC19 | This study |
| pZR15 | 7-kbp HpaII fragment containing pcaU and inserted in pUC19 | This study |
| pZR17 | 2,792-bp EcoRI fragment containing pcaU and inserted in pUC19 | This study |
| pZR18 | 2,618-bp HindIII-HpaII fragment containing pcaU and inserted in pUC19 | This study |
| pZR22 | 2,618-bp insert from pZR18 integrated in HindIII-SstI cleavage sites of pWH661+ | This study |
| pZR23 | 2,416-bp HindIII-EcoRI fragment from pZR17 inserted into HindIII-EcoRI cleavage sites of pUC18 | This study |
| pZR26 | SalI fragment containing lacZ-Kmr cassette from plasmid KOK6 inserted in Xho site of pZR22 | This study |
| pZR28 | SalI fragment containing lacZ-Kmr cassette from plasmid KOK6 inserted in Xho site of pZR92 | This study |
| pZR33 | pZR18 modified by ΔpcaU2 deletion (552-bp deletion of NcoI-MluI fragment) | This study |
| pZR91 | ΩSmrSpcr cassette in XhoI site of pZR9 | This study |
| pZR92 | 5,403-bp Sau3A insert from pZR9 in PstI-SstI site of pRK415 | This study |
a
See Fig. 3.