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[Preprint]. 2023 Nov 30:2023.02.04.527050. [Version 2] doi: 10.1101/2023.02.04.527050

Fig. 3. A subpopulation of NNAT+ beta cells develop during the early postnatal period in mice.

Fig. 3.

(a) Representative confocal microscopy of pancreatic cryosections from wild type mice on a C57BL/6J background of developmental stages from embryonic day 17.5 (E17.5) through the postnatal (P) period into adulthood. Sections were immunostained with antibodies against endogenous neuronatin (NNAT, green) and insulin (INS, red). Nuclei are visualised with DAPI and sections from P56 mice with constituent deletion of Nnat were used as an immunostaining control. Scale bar = 100μm (inset = 10μm) (n = 4-15 mice per timepoint, Kruskal-Wallace test with Dunn’s multiple comparisons). (b) Quantification of NNAT+ beta cells from images shown in a, expressed as NNAT/INS co-positive cells as a percentage of total INS-positive cells. (c) Representative confocal microscopy of pancreatic cryosections as in a from E17.5 and postnatal (P) day 14 mice (n = 18 and n = 5 mice, respectively) immunostained with antibodies against endogenous neuronatin (NNAT, green) and GCG or SST (both grey). Scale bar = 100μm. Representative images from three independent experiments and breeding pairs. * P < 0.05, ** P < 0.01, *** P < 0.001.