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. 2023 Nov 27;15(23):5607. doi: 10.3390/cancers15235607

Table 2.

List of FDA-approved biomarker-based multivariate Index assays for detection of EOC.

ROMA Test RMI OVA1 Test OVA2 (Overa)
FDA Approval (Year) 2011 N/A 2009 2016
Biomarker
• CA 125 x x x x
• HE4 x x
• B2M x
• ApoA-I x x
• FSH x
• TRF x
• Transferrin x x
Immunoassay
technique

ECLIA [Cobas 8000, Roche Diagnostics Scandinavia AB, Sweden]//
Magnetic bead assay
[xMAP bead-based technology (Luminex, Austin, Texas)]
ECLIA Cobas 6000 (Roche, Germany) /IVDMIA
[Quest Diagnostics Incorporated; Vermillion, (Austin, Texas)]
IVDMIA
[Quest Diagnostics Incorporated; Vermillion, (Austin, Texas)]
SP (%) >80 to 91 >80 to 90 <75 to 26 28–35
SN (%) >85 to 97 >87 to 97 >78 to 98 77–96
Ref. [40,52,60,65,67] [40,52,60,65,67] [36,44,52,54,60] [52,60,66]

Abbreviations: ROMA: Risk of Ovarian Malignancy Algorithm; Risk of Malignancy Index (RMI); FDA: Food and Drug Administration; CA 125: cancer antigen 125; HE4: human epididymis secretory protein 4; B2M: β2-microglobulin; ApoA-I: Apolipoprotein AI; FSH: follicle-stimulating hormone; Transthyretin (TRF); ECLIA: Electrochemiluminescence Immunoassay IVDMIA: In Vitro Diagnostic Multivariate Index Assay SP: Specificity; SN: Sensitivity. N/A: Not applicable; no data show the FDA approval year for RMI. The ‘x’ symbol indicates the inclusion of a biomarker in the ROMA test, RMI, OVA1 test, and OVA3 (Overa).