Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

[Preprint]. 2023 Dec 2:2023.10.05.561095. [Version 2] doi: 10.1101/2023.10.05.561095

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.

PMC Copyright notice

Figure 4. — A-F, Properties of SGN recordings are reported for regenerated synapses in co-cultures of SGNs and denervated organs of Corti, plated at ages P3-5, P7-8 or P10-11; and for native acutely excised P4-5 organs of Corti (P4-5 Native). A, Percentage of SGN recordings showing regenerated postsynaptic activity in response to hair cell stimulation is higher in P_7-8 or P_10-11 versus P_3-5 cultures. B, Distribution of ‘fast’, ‘slow ’and ‘mixed ’responses (as shown in Fig. 3) of regenerated synapses in comparison to P_4-5 Native synapses. Contribution of ‘fast responses is higher in regenerated synapses of P_7-8 or P_10-11 versus P_3-5 cultures, and 100% in P_4-5 Native synapses. C, EPSC waveform of a P_4-5 Native synapse (black) is slower that EPSC waveform from a P_3-5 regenerated synapse (pink; P_3-5 Reg). Individual waveforms are shown in grey and averaged in black or pink. Holding potential −79 mV. D-F, Comparison of EPSC waveform parameters for regenerated and native synapses. Each data point represents the median value from an individual recording. Numbers above each box indicated the number of SGN recordings used for analysis. Boxes represent the median (horizontal line), 10th and 90^th percentile. Whiskers represent maximum and minimum values of the distribution. *p<0.05, **p<0.01, n.s.: not significant, Kruskal–Wallis with Dunn’s post hoc test. D, 90-10% median decay time of native EPSCs (EPSCs^{(P4-5 Native)}; 1.81ms; n=7) were slower compared to regenerated EPSCs of all age conditions: EPSCs^{(P3-5 Reg)} (0.66ms; n=15; p<0.05); EPSCs^{(P7-8 Reg)} (0.60ms; n=8; p<0.01) and EPSCs^{(P10-11 Reg)} (0.58ms, n=6; p<0.01). However, decay time was not statistically different between regenerated EPSCs of all age conditions. E, The median amplitude of native EPSCs (EPSCs^{(P4-5 Native)}: 15.59pA; n=7) was similar compared to EPSCs^{(P3-5 Reg)} (18.72pA; n=15). However, EPSCs^{(P4-5 Native)} were significantly smaller when compared to EPSCs^{(P7-8 Reg)} (21.89pA; n=8; p<0.01) and to EPSCs^{(P10-11 Reg)} (25.77pA; n=6; p<0.05). F, The median area of native EPSCs (EPSCs^{(P4-5 Native)}; 46.00fC; n=7) was similar compared to regenerated EPSCs at all age conditions: EPSCs^{(P3-5 Reg)} (19.27fC; n=15), EPSCs^{(P7-8 Reg)} (30.25fC; n=8) and EPSCs^{(P10-11 Reg)} (24.00fC; n=6). Regenerated EPSCs also displayed similar area values at all age conditions.