The rrnB promoter region. (A) FIS sites I, II and III, UP elements, −10 and −35 hexamers, transcription start sites (+1), and transcripts from the p1 and p2 promoters (arrows) are indicated. (B) DNA sequence of the region containing FIS sites I, II, and III in rrnBp1. G residues protected by FIS against methylation by DMS (Fig. 2 and reference 8) are indicated with asterisks. Lines indicate sequences protected by FIS against DNase I cleavage (48).