FIG. 6.

FIS-dependent activation of rrnBp₁ during outgrowth from stationary phase. (A) Primer extension products from wild-type and Δ72 mutant rrnBp₁ promoters (from RLG1350 and RLG1831, respectively) were measured at the indicated times during the first 60 min following dilution of cells from stationary phase into fresh LB. Equal volumes of culture were used for all time points, and the data were subsequently normalized for culture density (B). Primer extension products from rrnBp₁ promoters, extending to +1, and recovery marker products (from RLG1829 cells containing an rrnBp₁ promoter, extending to +50) are indicated. (B) Activities of wild-type and Δ72 rrnBp₁ promoters, normalized for recovery and increasing culture density (arbitrary units). Results from four experiments similar to that shown in panel A were quantified and averaged. Error bars represent standard deviations. The growth curve (OD₆₀₀) is shown from one strain in one experiment for clarity, but the growth curves from both strains in all four experiments were very similar.