TABLE 1.
Strains, λ lysogens, and plasmids used
| Strain, λ lysogen, or plasmid | Genotype | Reference |
|---|---|---|
| Strains | ||
| MG1655 | 2 | |
| RLG851 | MG1655 ΔlacX74 (=CAG4000) | 48 |
| LL309 | F′ lacIq ZΔM15Y+pro+/Δ(lac/pro) thi nalA supE Bfer | 24 |
| RLG1863 | RLG851 fis::kan-767 | This work |
| RLG911 | RLG851(pSL7) | This work |
| RLG912 | RLG851(pAJ4) | 8 |
| RLG921 | RLG1863(pSL7) | This work |
| RLG1819 | RLG851(pRLG1819) | This work |
| RLG1820 | RLG851(pRLG1820) | This work |
| λ lysogens | ||
| RLG1350 | RLG851/λ system I rrnBp1 −88 to +1-lacZ | 48 |
| RLG1816 | LL309/λ system II rrnBp1 −88 to −37, lac −36 to +57-lacZ | This work |
| RLG1817 | LL309/λ system II rrnBp1 −88 to −37 Δ72, lac −36 to +57-lacZ | 47 |
| RLG1823 | LL309 fis::kan 767/λ system II rrnBp1 −88 to −37, lac −36 to +57-lacZ | This work |
| RLG1824 | LL309 fis::kan 767/λ system II rrnBp1 −88 to −37 Δ72, lac −36 to +57-lacZ | This work |
| RLG1829 | RLG851/λ system I rrnBp1 −88 to +50-lacZ | This work |
| RLG1831 | MG1655/λ system I rrnBp1 −88 to +1 Δ72-lacZ | This work |
| Plasmids | ||
| pSL6 | 21 | |
| pRLG770 | 48 | |
| pSL7 | pSL6 (rrnBp1 −154 to +50) | 21 |
| pAJ4 | pSL6 (rrnBp1 −154 to +50 Δ72) | 8 |
| pRLG1819 | pRLG770 (rrnBp1 −88 to −37, lac −36 to +57) | This work |
| pRLG1820 | pRLG770 (rrnBp1 −88 to −37 Δ72, lac −36 to +57) | This work |