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. 2023 Nov 22;12(23):2687. doi: 10.3390/cells12232687

Figure 2.

Figure 2

The 3′UTR of RRAS2 frequently bears somatic mutations at positions +26, +53, and +180 from the stop codon. (A) Schematic representation of the site of rs8570 in the 3′UTR of RRAS2 mRNA, 124 nucleotides downstream of the stop codon. The SNP position is indicated in a box and the canonical nucleotide (G) at that position is in red. Other sites in the 3′UTR (+26, +53, and +180) at which somatic mutations are found in our cohort of CLL patients are indicated with the canonical nucleotides. (B–D) Dedicated qPCRs were used to determine the ratio between the alternate and canonical nucleotides for each of the three sites found mutated in the cohort (n = 270) of CLL blood samples and represented in the y-axis. The sample number is indicated in the x-axis. The mean value of all ratios is indicated with a solid green line and the median with a broken green line. Samples with ratios above the median were considered as bearing the alternate allele at least in heterozygosity. Numbers beside the red dots point out to the samples containing simultaneously the three mutations. (E) Stacked bar plot showing the distribution of CLL blood samples according to the presence of somatic mutations at positions +26, +53, and +180 in isolation or in combinations.