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[Preprint]. 2023 Nov 29:2023.11.28.569058. [Version 1] doi: 10.1101/2023.11.28.569058

Figure 2. Phosphorylated p62 accumulates prominently in ATG9A KO HEK293T cells.

Figure 2.

(A) Western blot demonstrating the defects of p62 degradation in ATG5, ATG9A, and ATG13 KO cell lines. (B) Quantification of p62 immunoblot signal normalized to actin from 3 replicates. Error bars represent SD. P-values were calculated using a two-tailed student t-test. (C) Immunoblot showing the buildup of phospho-p62 in the indicated ATG protein KO cell lines. (D-E) Quantification (as done in panels B-C) of pS349 and pS403 on p62 from 3 biological replicates of immunoblots as shown in panel D. Error bars represent SD. P-values were calculated using a two-tailed student t-test. (F-G) Images depicting the accumulation of pS349 or pS403 p62 in WT and ATG9A KO HEK293T cells (scale bars = 10 μm).